Structural characterization of AtmS13, a putative sugar aminotransferase involved in indolocarbazole AT2433 aminopentose biosynthesis

Academic Article


  • AT2433 from Actinomadura melliaura is an indolocarbazole antitumor antibiotic structurally distinguished by its unique aminodideoxypentose-containing disaccharide moiety. The corresponding sugar nucleotide-based biosynthetic pathway for this unusual sugar derives from comparative genomics where AtmS13 has been suggested as the contributing sugar aminotransferase (SAT). Determination of the AtmS13 X-ray structure at 1.50-Å resolution reveals it as a member of the aspartate aminotransferase fold type I (AAT-I). Structural comparisons of AtmS13 with homologous SATs that act upon similar substrates implicate potential active site residues that contribute to distinctions in sugar C5 (hexose vs. pentose) and/or sugar C2 (deoxy vs. hydroxyl) substrate specificity.
  • Authors

    Digital Object Identifier (doi)

    Pubmed Id

  • 27093172
  • Author List

  • Singh S; Kim Y; Wang F; Bigelow L; Endres M; Kharel MK; Babnigg G; Bingman CA; Joachimiak A; Thorson JS
  • Start Page

  • 1547
  • End Page

  • 1554
  • Volume

  • 83
  • Issue

  • 8