Crystal structure of SsfS6, the putative C-glycosyltransferase involved in SF2575 biosynthesis

Academic Article


  • The molecule known as SF2575 from Streptomyces sp. is a tetracycline polyketide natural product that displays antitumor activity against murine leukemia P388 in vivo. In the SF2575 biosynthetic pathway, SsfS6 has been implicated as the crucial C-glycosyltransferase (C-GT) that forms the C-C glycosidic bond between the sugar and the SF2575 tetracycline-like scaffold. Here, we report the crystal structure of SsfS6 in the free form and in complex with TDP, both at 2.4 Å resolution. The structures reveal SsfS6 to adopt a GT-B fold wherein the TDP and docked putative aglycon are consistent with the overall C-glycosylation reaction. As one of only a few existing structures for C-glycosyltransferases, the structures described herein may serve as a guide to better understand and engineer C-glycosylation. © 2013 Wiley Periodicals, Inc.
  • Authors

    Digital Object Identifier (doi)

    Pubmed Id

  • 27982618
  • Author List

  • Wang F; Zhou M; Singh S; Yennamalli RM; Bingman CA; Thorson JS; Phillips GN
  • Start Page

  • 1277
  • End Page

  • 1282
  • Volume

  • 81
  • Issue

  • 7