A pipeline of integrating transcriptome and interactome to elucidate central nodes in host-pathogens interactions

Academic Article


  • Investigating the complexity of host-pathogen interactions is challenging. Here, we outline a pipeline to identify important proteins and signaling molecules in human-viral interactomes. Firstly, we curate a comprehensive human interactome. Subsequently, we infer viral targets and transcriptome-specific human interactomes (VTTSHI) for papillomavirus and herpes viruses by integrating viral targets and transcriptome data. Finally, we reveal the common and shared nodes and pathways in viral pathogenesis following network topology and pathway enrichment analyses. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).
  • Authors

    Published In

  • STAR Protocols  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 26580570
  • Author List

  • Kumar N; Mishra B; Mukhtar MS
  • Volume

  • 3
  • Issue

  • 3