Expression of hemolytically active murine fourth component of complement in transfected L cells

Academic Article


  • The S region of the murine major histocompatibility complex contains two related genes (C4-X and C4-Y), one of which encodes the fourth component of complement (C4). We have introduced cloned C4-X and C4-Y genes into L cells. C4-X transfectants contained no detectable C4-related RNA, whereas C4-Y transfectants contained large amounts of C4-related RNA. Culture supernatants of C4-Y transfectants contained processed C4 which was indistin-guishable from macrophage-derived C4. The L-cell-derived C4 restored hemolytic activity to C4-deficient serum, served as a substrate for C1s, and underwent autolytic cleavage suggesting the presence of an intramolecular thiolester bond. These experiments identify C4-Y as the functional C4 gene in H-2d mice and indicate that L cells can process pro-C4 correctly. © 1984.
  • Published In

  • Cell  Journal
  • Digital Object Identifier (doi)

    Author List

  • Chaplin DD; Sackstein R; Perlmutter DH; Weis JH; Kruse TA; Coligan J; Colten HR; Seidman JG
  • Start Page

  • 569
  • End Page

  • 576
  • Volume

  • 37
  • Issue

  • 2