Germline organization of the murine T cell receptor β-chain genes

Academic Article

Abstract

  • The complete germline organization of the β-chain genes of the murine T cell receptor was elucidated in order to obtain the structural basis for understanding the mechanisms of somatic DNA rearrangements. Twenty of the 22 known variable (V ) genes are clustered within 250 kilobases of DNA 5′ to the constant region (C ) genes. These V genes share the same transcriptional orientation as the diversity (D ), joining (J ), and C genes, which implies that chromosomal deletion is the mechanism for most V to D -J rearrangements. Within this V cluster, the distance between the most proximal V gene and the D -J -C cluster is 320 kilobases, as determined by field-inversion gel electrophoresis. The large distance between V and D , relative to that between D and J , may have significant implications for the ordered rearrangement of the T cell receptor β-chain genes. β β β β β β β β β β β β β β β β β β
  • Published In

  • Science  Journal
  • Digital Object Identifier (doi)

    Author List

  • Chou HS; Nelson CA; Godambe SA; Chaplin DD; Loh DY
  • Start Page

  • 545
  • End Page

  • 548
  • Volume

  • 238
  • Issue

  • 4826