Serotonin depletion hampers survival and proliferation in neurospheres derived from adult neural stem cells

Academic Article

Abstract

  • Serotonin (5-HT) and the serotonergic system have recently been indicated as modulators of adult hippocampal neurogenesis. In this study, we evaluated the role of 5-HT on the functional features in neurospheres derived from adult neural stem cells (ANSC). We cultured neurospheres derived from mouse hippocampus in serum-free medium containing epidermal (EGF) and type-2 fibroblast growth factor (FGF2). Under these conditions ANSC expressed both isoforms of tryptophane-hydroxylase (TPH) and produced 5-HT. Blocking TPH function by para-chlorophenylalanine (PCPA) reduced ANSC proliferation, which was rescued by exogenous 5-HT. 5-HT action on ANSC was mediated predominantly by the serotonin receptor subtype 5-HT1A and, to a lesser extent, through the 5-HT2C (receptor) subtype, as shown by selectively antagonizing these receptors. Finally, we documented a 5-HT-induced increase of ANSC migration activity. In summary, we demonstrated a powerful serotonergic impact on ANSC functional features, which was mainly mediated by 5-HT1A receptors. © 2010 Nature Publishing Group All rights reserved.
  • Authors

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    Digital Object Identifier (doi)

    Author List

  • Benninghoff J; Gritti A; Rizzi M; Lamorte G; Schloesser RJ; Schmitt A; Robel S; Genius J; Moessner R; Riederer P
  • Start Page

  • 893
  • End Page

  • 903
  • Volume

  • 35
  • Issue

  • 4