Hyperintensity of integrin-targeted fluorescence agent IntegriSense750 accurately predicts flap necrosis compared to Indocyanine green

Academic Article


  • Background: Flap necrosis is a feared complication of reconstructive surgery. Current methods of prediction using Indocyanine green (ICG) lack specificity. IntegriSense750 is a fluorescence agent that binds sites of vascular remodeling. We hypothesized that IntegriSense750 better predicts flap compromise compared to ICG. Methods: Fifteen mice underwent lateral thoracic artery axial flap harvest. Mice received an injection of ICG (n = 7) or IntegriSense750 (n = 8) daily from postoperative days (POD) 0–3 and were imaged daily. Mean signal-to-background ratios quantified the change in fluorescence as necrosis progressed. Results: Mean signal-to-background ratio was significantly higher for IntegriSense750 compared to ICG on POD0 (1.47 ± 0.17 vs. 0.86 ± 0.21, p = 0.01) and daily through POD3 (2.12 ± 0.70 vs. 0.96 ± 0.29, p < 0.001). Conclusions: IntegriSense750 demonstrates increased signal-to-background ratio at areas of flap distress compared to ICG which may increase identification of flap necrosis and improve patient outcomes.
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    Author List

  • Hicks MD; Ovaitt AK; Fleming JC; Sorace AG; Song PN; Mansur A; Hartman BS YE; Rosenthal EL; Warram JM; Thomas CM
  • Start Page

  • 134
  • End Page

  • 142
  • Volume

  • 44
  • Issue

  • 1