Promotion of proliferation of murine BALB/c 3T3 fibroblasts mediated by nitric oxide at lower concentrations

Academic Article


  • This study showed that nitric oxide (NO)-generating S-nitrosothiols, S-nitroso-N-acetylpenicillamine (SNAP) and S-nitrosoglutathione (GSNO), increased proliferation of BALB/c 3T3 (clone A31-1-1) fibroblasts in vitro. Treatment with SNAP at a relatively low concentration (0.005-0.02 mM) induced an increase in cell number compared to control. SNAP (0.005-0.02 mM) and GSNO (0.025-0.05 mM) both showed an increase of [3H]thymidine incorporation in exponentially growing cells in a dose-dependent manner. The maximal effect was observed at about 40 and 90% above control at 0.02 mM and 0.05 mM, respectively. The increase induced by 0.02 mM SNAP was abolished by the addition of 0.01 mM oxyhemoglobin, a scavenger of NO. [3H]Thymidine incorporation in stationary cells was also increased by SNAP. In addition, 0.02 mM SNAP produced a 1.8-3.2-fold increase of thymidine kinase activity in exponentially growing cells.
  • Authors

    Published In

  • IUBMB Life  Journal
  • Digital Object Identifier (doi)

    Author List

  • Du M; Islam MM; Lin L; Ohmura Y; Moriyama Y; Fujimura S
  • Start Page

  • 625
  • End Page

  • 631
  • Volume

  • 41
  • Issue

  • 3