Surface treatment of pure and PEG-4000 blended fibroin films and their characterizations as matrices for in vitro fibroblast culture.

Academic Article

Abstract

  • This study reports the effects of treatment with various concentrations of organic solvents for varying time points on matrices of fibroin, a silk protein isolated from the mulberry silkworm, Bombyx mori, which in native form has been extensively used in tissue engineering. Treatment of pure fibroin as well as polyethylene glycol- blended films with 90% organic solvent for 60 min induces optimal surface hydrophobicity and maximum conversion of the secondary structure from random coil to beta sheet. Long-term cell viability studies reveal that methanol and isopropanol-treated pure and blended films support cell adhesion, proliferation, and viability.
  • Authors

    Published In

    Keywords

  • 2-Propanol, Animals, Biocompatible Materials, Cell Adhesion, Cell Line, Cell Survival, Fibroblasts, Fibroins, Mice, Polyethylene Glycols, Silk, Surface Properties
  • Digital Object Identifier (doi)

    Pubmed Id

  • 15969719
  • Author List

  • Acharya C; Dutta A; Kundu SC
  • Start Page

  • 497
  • End Page

  • 517
  • Volume

  • 23
  • Issue

  • 6