GTPase activating protein function of p85 facilitates uptake and recycling of the β1 integrin

Academic Article

Abstract

  • β1-containing adhesions at the plasma membrane function as dynamic complexes to provide bidirectional communication between the cell and its environment, yet commonly are used by pathogens to gain host cell entry. Recently, the cholesterol-lowering drug simvastatin was found to inhibit host invasion through β1-containing adhesion complexes. To better understand the regulatory mechanisms controlling adhesion formation and uptake and the use of these complexes by Staphylococcus aureus, the primary etiologic agent in sepsis, bacteremia and endocarditis, we investigated the mechanism of inhibition by simvastatin. In response to simvastatin, adhesion complexes diminished as well as β1 trafficking to the plasma membrane required to initiate adhesion formation. Simvastatin stimulated CDC42 activation and coupling to p85, a small-guanosine triphosphatase (GTPase) activating protein (GAP), yet sequestered CDC42 coupled to p85 within the cytosol. Loss of p85 GAP activity through use of genetic strategies decreased host cell invasion as well as β1 trafficking. From these findings, we propose a mechanism whereby p85 GAP activity localized within membrane compartments facilitates β1 trafficking. By sequestering p85 within the cytosol, simvastatin restricts the availability and uptake of the receptor used by pathogenic strains to gain host cell entry. © 2009 Elsevier Inc. All rights reserved.
  • Digital Object Identifier (doi)

    Author List

  • Stankiewicz TE; Haaning KL; Owens JM; Jordan AS; Gammon K; Bruns HA; McDowell SA
  • Start Page

  • 443
  • End Page

  • 448
  • Volume

  • 391
  • Issue

  • 1