Nanodisc reconstitution of flavin mononucleotide binding domain of cytochrome-P450-reductase enables high-resolution NMR probing

Academic Article

Abstract

  • Cytochrome-P450-reductase transfers electrons to cytochrome-P450 through its flavin mononucleotide binding domain (FBD). Despite the importance of membrane-anchoring for FBD function, studies have focused on its soluble domain lacking the transmembrane-domain. Here we demonstrate that the reconstitution of FBD in nanodiscs enables high-resolution NMR measurements and renders a stable conformation.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Krishnarjuna B; Yamazaki T; Anantharamaiah GM; Ramamoorthy A
  • Start Page

  • 4819
  • End Page

  • 4822
  • Volume

  • 57
  • Issue

  • 39