A New Class of Small RNP (α-RNP) Containing Antisense RNA in K-562 Cell Line. III. DNA-Binding Activity of Nuclear α-RNP

Academic Article

Abstract

  • DNA-binding activity of small nuclear α-RNP identified in acid-soluble fraction of chromatin of human proerythroleukemic cell line K-562 was studied using the technique of gel retardation. We found that nuclear α-RNP isolated from K-562 cells through treatment with dimethylsulfoxide, an agent inducing differentiation, acquire a capacity to specific interaction with Alu repeats of DNA leading to the formation of α-RNP-Alu-DNA complexes; nuclear α-RNP from cells that were not treated with dimethylsulfoxide do not show such capacity, although they are tightly bound with chromatin in the cell. Thus, the capacity of nuclear α-RNP to direct interaction with DNA Alu repeats appearing after the induction of K-562 cells to differentiation along erythroid pathway is an inducible property. We discuss hypothesis about the involvement of nuclear α-RNP in the control of expression of inducible genes at the level of chromatin and interaction with DNA.
  • Author List

  • Konstantinova IM; Vetzcker R; Petukhova OA; Chesnokov IN; Volkova IV; Turoverova LV; Ermolaeva YB; Gause LN
  • Start Page

  • 176
  • End Page

  • 177
  • Volume

  • 28
  • Issue

  • 3