Binding specificity of human nuclear protein interacting with the Alu-family DNA repeats

Academic Article

Abstract

  • We have demonstrated earlier that human cells contain nuclear protein interacting with conserved GC-rich sequence motifs of human Alu-family DNA repeats. One of these sequences is located in the region between elements A and B of bipartite RNA polymerase III promoter of Alu (AB-region). In this study we have used a DNase I footprinting assay with an Alu restriction subfragment covering AB-region, as well as a gel mobility shift assay with appropriate synthetic oligonucleotides to analyse in more detail the interaction of the protein with AB-region. We have also used antibodies raised against a zinc-finger peptide to examine the presence of a zinc-finger in the Alu-binding protein. The results indicate that AGG triplets may be important for high-affinity binding of the protein to DNA, and that the Alu-binding protein is a zinc-finger protein. © 1991.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 20448521
  • Author List

  • Chesnokov I; Bozhkov V; Popov B; Tomilin N
  • Start Page

  • 613
  • End Page

  • 619
  • Volume

  • 178
  • Issue

  • 2