Out-of-equilibrium CO2/HCO3- solutions and their use in characterizing a new K/HCO3 cotransporter

Academic Article

Abstract

  • In typical physiological solutions, CO is in equilibrium with HCO and H (CO + H O⇆HCO + H ). Because one cannot independently alter CO and HCO concentrations and pH, it is impossible to distinguish between the effects of CO and HCO on physiological processes. Here we describe a continuous-flow, rapid-mixing approach for generating out-of-equilibrium CO / HCO solutions with a physiological pH and CO (but little HCO ), or pH and HCO (but little CO ). We have exploited these out-of-equilibrium solutions to introduce HCO exclusively to either the outside or inside of a squid giant axon, and verify the presence of a new K/HCO cotransporter. The out-of-equilibrium approach could be useful in a variety of applications for independently controlling CO and HCO concentrations and pH. © 2002 Nature Publishing Group. 2 3 2 2 3 2 3 2 3 2 3 2 3 3 2 3 3 2 3 - + - + - - - - - - -
  • Authors

    Published In

  • Nature  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 13163599
  • Author List

  • Zhao J; Hogan EM; Bevensee MO; Boron WF
  • Start Page

  • 636
  • End Page

  • 639
  • Volume

  • 374
  • Issue

  • 6523