Microsome-dependent methylation of erythrocyte proteins by dimethylnitrosamine

Academic Article

Abstract

  • The precarcinogen, N-[methyl-14C]dimethylnitro-samine, rat liver microsomes and NADPH were incubated in the presence of intact human erythrocytes. Uptake of radioactivity into erythrocytes was time-dependent and analysis of an acid hydrolysate of the erythrocyte proteins showed several radioactive peaks which were identified as 3-N-methyl- and 1-N-methyl-histidine, and S-methyl-cysteine. Since no binding occurs in the absence of microsomes, the alkylating species from dimethylnitrosamine metabolism can clearly penetrate the erythrocyte plasma membrane. © 1981 IRL Press Limited.
  • Authors

    Published In

  • Carcinogenesis  Journal
  • Digital Object Identifier (doi)

    Author List

  • Kim S; Paik WK; Choi J; Lotlikar PD; Magee PN
  • Start Page

  • 179
  • End Page

  • 182
  • Volume

  • 2
  • Issue

  • 3