Dietary phytochemicals induce p53- and caspase-independent cell death in human neuroblastoma cells

Academic Article

Abstract

  • Neuroblastoma (NB) is the most prevalent pediatric solid tumor and a leading cause of cancer-related death in children. In the present study, a novel cytotoxic role for the dietary compounds, curcumin, andrographolide, wedelolactone, dibenzoylmethane, and tanshinone IIA was identified in human S-type NB cells, SK-N-AS and SK-N-BE(2). Mechanistically, cell death appeared apoptotic by flow cytometry; however, these effects proceeded independently from both caspase-3 and p53 activation, as assessed by both genetic (shRNA) and pharmacological approaches. Notably, cell death induced by both curcumin and andrographolide was associated with decreased NFκB activity and a reduction in Bcl-2 and Bcl-xL expression. Finally, curcumin and andrographolide increased cytotoxicity following co-treatment with either cisplatin or doxorubicin, two chemotherapeutic agents widely used in the clinical management of NB. Coupled with the documented safety in humans, dietary compounds may represent a potential adjunct therapy for NB. © 2011 ISDN.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Sukumari-Ramesh S; Bentley JN; Laird MD; Singh N; Vender JR; Dhandapani KM
  • Start Page

  • 701
  • End Page

  • 710
  • Volume

  • 29
  • Issue

  • 7