The Mycobacterium tuberculosis 71‐kDa heat‐shock protein induces proliferation and cytokine secretion by murine gut intraepithelial lymphocytes

Academic Article


  • Murine intraepithelial lymphocytes (IEL) respond poorly to T cell mitogens and to monoclonal antibody stimulation of T cell receptor (TCR)‐ and CD3‐associated molecules. In contrast, we found that a soluble extract of Mycobacterium tuberculosis (Mtb), but not purified protein derivative of tuberculin, induced significant proliferative responses in IEL cultures. The active component was apparently a heat shock protein (HSP), since recombinant 71‐kDa HSP from Mtb induced IEL to proliferate, while 65‐kDa HSP from M. bovis and M. leprae did not. Both α/β and α/δ TCR‐enriched IEL gave proliferative responses to 71‐kDa HSP. Further, culture supernatants from IEL stimulated with 71‐kDa HSP contained elevated levels of interleukin‐(IL)‐3/granulocyte‐macrophage colony‐stimulating factor, interferon‐y and IL‐6, but not IL‐2, IL‐4, IL‐5 or transforming growth factor‐β Finally, several IEL T cell clones have been maintained for up to 6 weeks, when stimulated with 71‐kDa HSP, IL‐2 and feeder cells. Our results show that the 71‐kDa HSP of Mtb induces IELT cells to divide and to secrete cytokines and this may offer a model for cloning and study of IEL T cells in vitro. Copyright © 1993 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
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    Author List

  • Beagley KW; Fujihashi K; Black CA; Lagoo AS; Yamamoto M; McGhee JR; Kiyono H
  • Volume

  • 23