Mechanisms for mucosal immunogenicity and adjuvancy of Escherichia coli labile enterotoxin

Academic Article

Abstract

  • Escherichia coli labile toxin (LT) was assessed as mucosal immunogen and as adjuvant for tetanus toxoid (TT) in mice. After oral administration of LT, C57BL/6 (H-2b) and BALB/c (H-2(d)) mice were high mucosal and serum antibody responders, while C3H/HeN (H-2(k)) mice were low responders. High responders exhibited mainly serum IgG (including IgG1, IgG2a, and IgG2b), as well as IgM and IgA, while mucosal responses were IgA. Analysis of LT-B-specific CD4+ T helper (Th) cells from Peyer's patches (PP) or from spleen revealed a mixed Th1 (interferon-γ) and Th2 (interleukin-4 and -5) cell pattern. Oral LT given with TT induced TT-specific response patterns identical to LT-B. Analysis of mRNA from TT-specific PP CD4+ Th cells also revealed a mixed Th1- and Th2-type response. Thus, antibody response profiles induced by LT are regulated by both CD4+ Th1 and Th2 cell types.
  • Digital Object Identifier (doi)

    Author List

  • Takahashi I; Marinaro M; Kiyono H; Jackson RJ; Nakagawa I; Fujihashi K; Hamada S; Clements JD; Bost KL; McGhee JR
  • Start Page

  • 627
  • End Page

  • 635
  • Volume

  • 173
  • Issue

  • 3