© 2020, Springer Science+Business Media, LLC, part of Springer Nature. We evaluated outcomes from a healthy eating/nutrition label interpretation intervention among Latinx immigrant mothers and their daughters, aged 9–12 years, in Alabama. Between May 2013–October 2017, this cluster randomized controlled trial assigned 299 mother-daughter dyads to either a healthy eating (intervention) or HPV vaccination (control) study arm. Participants attended four group sessions delivered in Spanish by Lay Health Educators covering portion sizes, healthy eating/cooking strategies, and nutrition label reading/interpretation. An individual session in participants’ homes reviewed pantries and developed healthy eating plans. Identical interviewer-administered surveys were completed at baseline and 7-month follow-up by both study arms. Retention rate at follow-up was 93.4% in intervention arm (92.6% in control arm). Positive changes in healthy eating behaviors and proficiency in nutrition label interpretation were assessed. Adjusting for marital status, employment status, and health insurance coverage status, when compared to controls, mothers in the intervention arm had greater odds of increasing daily fruit and vegetable consumption (OR 3.66, 95% CI 2.14–6.27, p < 0.001), decreasing weekly fried food intake (OR 4.3, 95% CI 2.3–8.04, p < 0.001), decreasing daily sweetened beverages (OR 2.07, 95% CI 1.22–3.52, p < 0.01), increasing frequency of reading nutrition labels (OR 12.58, 95% CI 6.81–23.22, p < 0.001), and correctly interpreting nutrition labels (OR 4.45, 95% CI 2.64–7.48, p < 0.001). Significant positive changes in targeted behaviors were not observed among daughters. A community-based, culturally relevant intervention that includes nutrition label interpretation can positively influence eating habits among Latinx immigrant mothers.