Detection of hepatitis B surface protein by means of laser spectroscopy

Academic Article

Abstract

  • The purpose of this experiment is to detect small concentrations of purified (HBsAg) hepatitis B surface antigen in a buffered salt solution. Such possibilities could be provided by means of optical spectroscopy, which includes laser-induced self-luminescence and Raman spectroscopy. Luminescence spectroscopy under selective laser excitation is a very sensitive method to determine the presence and concentration of proteins in solutions. In the experiment, UV laser light at 266 nm, which corresponds to the absorption band of Tryptophan and Tyrosine groups, is used. As a detection system TE-cooled CCD camera attached to 0.15-m spectrograph is used. Such combination provided extremely high sensitivity and relatively good spectral resolution (≥4 cm-1).
  • Authors

    Author List

  • Dergachev A; Kudryavtsev A; Mirov S; McCracken M
  • Start Page

  • 158
  • End Page

  • 159