The aim of this study was to determine the effect a separated instrument has on the time required for bacterial penetration of obturated root canals. Twenty-six extracted human mandibular premolars with single canals were used in the study. Group 1 consisted of teeth that contained a separated size 40 Profile rotary file and were obturated with gutta-percha and zinc oxide eugenol sealer to the level of the separated file. Group 2 consisted of teeth that were similarly obturated, but without a separated file. The negative control canals were obturated and had the entire root surface sealed with nail polish. The positive controls were obturated without sealer. Streptococcus sanguis was placed in the access chamber daily, and penetration was determined when turbidity was noted in the culture broth. The results showed no significant difference between the two experimental groups.
