Objective: To determine the ideal angle for insertion of laparoscopic instruments at Palmer's point. Study Design: Abdominal magnetic resonance images were reviewed for 75 women between ages 18 and 50 years old. The distance from the skin to the retroperitoneal structures were determined perpendicular to the spine and angled 45° caudally. Results: When instruments are inserted perpendicular to the skin in the axial plane and peritoneum perpendicular to the spine, the distance from skin to posterior was 10.0 ± 0.2 cm and to the aorta was 11.3 ± 0.2 cm. If instruments are inserted at an angle 45° caudally, this distance increased to 16.6 ± 0.2 cm. Conclusion: When inserting laparoscopic instruments at Palmer's point, insertion perpendicular to the skin in the axial plane and angled 45° caudally in relation to the spine offers an increased margin of safety compared with insertion perpendicular to the spine, particularly in thin women. © 2009 Mosby, Inc. All rights reserved.