Constitutive role of the Fanconi anemia D2 gene in the replication stress response.

Academic Article

Abstract

  • In response to DNA cross-linking damage, the Fanconi anemia (FA) core complex activates the FA pathway by monoubiquitinating Fanconi anemia complementation group D2 (FANCD2) for the initiation of the nucleolytic processing of the DNA cross-links and stabilization of stalled replication forks. Given that all the classic FA proteins coordinately monoubiquitinate FANCD2, it is unclear why losses of individual classic FA genes yield varying cellular sensitivities to cross-linking damage. To address this question, we generated cellular knock-out models of FA core complex components and FANCD2 and found that FANCD2-null mutants display higher levels of spontaneous chromosomal damage and hypersensitivity to replication-blocking lesions than Fanconi anemia complementation group L (FANCL)-null mutants, suggesting that FANCD2 provides a basal level of DNA protection countering endogenous lesions in the absence of monoubiquitination. FANCD2's ubiquitination-independent function is likely involved in optimized recruitment of nucleolytic activities for the processing and protection of stressed replication forks. Our results reveal that FANCD2 has a ubiquitination-independent role in countering endogenous levels of replication stress, a function that is critical for the maintenance of genomic stability.
  • Authors

    Published In

    Keywords

  • DNA damage, DNA repair, DNA replication, Fanconi anemia, gene knock-out, genomic instability, nucleases, replication stress, DNA Damage, DNA Replication, Fanconi Anemia Complementation Group D2 Protein, Gene Knock-In Techniques, Gene Knockdown Techniques, Genomic Instability, HeLa Cells, Humans, Stress, Physiological, Ubiquitination
  • Digital Object Identifier (doi)

    Pubmed Id

  • 16997521
  • Author List

  • Tian Y; Shen X; Wang R; Klages-Mundt NL; Lynn EJ; Martin SK; Ye Y; Gao M; Chen J; Schlacher K
  • Start Page

  • 20184
  • End Page

  • 20195
  • Volume

  • 292
  • Issue

  • 49