Selective fluorescent labeling of S-nitrosothiols (S-FLOS): A novel method for studying S-nitrosation

Academic Article

Abstract

  • Protein S-nitrosation is a reversible post-translation modification critical for redox-sensitive cell signaling that is typically studied using the Biotin Switch method. This method and subsequent modifications usually require avidin binding or Western blot analysis to detect biotin labeled proteins. We describe here a modification of the Biotin Switch assay that eliminates the need for Western blot or avidin enrichment protocols and allows direct comparison of the S-nitrosation state proteins from two different samples in the same gel lane or on the same 2D gel. This S-FLOS method offers detection, identification and quantification of S-nitrosated proteins, with the potential for site-specific identification of nitrosation events. © 2008 Elsevier Inc. All rights reserved.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Santhanam L; Gucek M; Brown TR; Mansharamani M; Ryoo S; Lemmon CA; Romer L; Shoukas AA; Berkowitz DE; Cole RN
  • Start Page

  • 295
  • End Page

  • 302
  • Volume

  • 19
  • Issue

  • 3