Evaluation of the fluorescamine test for monitoring completion of coupling on nitrochloromethylated resin

Academic Article


  • The fluorescamine test was found to be unsuitable for monitoring the completion of coupling for solid phase peptide synthesis when nitrochloromethylated polystyrene-divinylbenzene resin was employed as a solid support. This limitation of the normally useful fluorescamine test resulted from the nitrated polymer completely quenching the fluorescence from the fluorophor-resin. The ninhydrin color test, which was unaffected by quenching effects, was found to be a suitable method by which reaction completeness can be monitored on a nitrochloromethylated resin. © 1976.
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    Digital Object Identifier (doi)

    Author List

  • Smith JA
  • Start Page

  • 477
  • End Page

  • 483
  • Volume

  • 74
  • Issue

  • 2