Assembly of productive T cell receptor δ variable region genes exhibits allelic inclusion

Academic Article

Abstract

  • The generation of a productive 'in-frame' T cell receptor β (TCR β), immunoglobulin (Ig) heavy (H) or Ig light (L) chain variable region gene can result in the cessation of rearrangement of the alternate allele, a process referred to as allelic exclusion. This process ensures that most αβ T cells express a single TCR β chain and most B cells express single IgH and IgL chains. Assembly of TCR α and TCR γ chain variable region genes exhibit allelic inclusion and αβ and γδ T cells can express two TCR α or TCR γ chains, respectively. However, it was not known whether assembly of TCR δ variable regions genes is regulated in the context of allelic exclusion. To address this issue, we have analyzed TCR δ rearrangements in a panel of mouse splenic γδ T cell hybridomas. We find that, similar to TCR α and γ, variable region genes, assembly of TCR δ variable region genes exhibits properties of allelic inclusion. These findings are discussed in the context of γδ T cell development and regulation of rearrangement of TCR δ genes.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Sleckman BP; Khor B; Monroe R; Alt FW
  • Start Page

  • 1465
  • End Page

  • 1471
  • Volume

  • 188
  • Issue

  • 8