DNA Breaks and End Resection Measured Genome-wide by End Sequencing

Academic Article

Abstract

  • DNA double-strand breaks (DSBs) arise during physiological transcription, DNA replication, and antigen receptor diversification. Mistargeting or misprocessing of DSBs can result in pathological structural variation and mutation. Here we describe a sensitive method (END-seq) to monitor DNA end resection and DSBs genome-wide at base-pair resolution in vivo. We utilized END-seq to determine the frequency and spectrum of restriction-enzyme-, zinc-finger-nuclease-, and RAG-induced DSBs. Beyond sequence preference, chromatin features dictate the repertoire of these genome-modifying enzymes. END-seq can detect at least one DSB per cell among 10,000 cells not harboring DSBs, and we estimate that up to one out of 60 cells contains off-target RAG cleavage. In addition to site-specific cleavage, we detect DSBs distributed over extended regions during immunoglobulin class-switch recombination. Thus, END-seq provides a snapshot of DNA ends genome-wide, which can be utilized for understanding genome-editing specificities and the influence of chromatin on DSB pathway choice.
  • Authors

    Published In

  • Molecular Cell  Journal
  • Digital Object Identifier (doi)

    Author List

  • Canela A; Sridharan S; Sciascia N; Tubbs A; Meltzer P; Sleckman BP; Nussenzweig A
  • Start Page

  • 898
  • End Page

  • 911
  • Volume

  • 63
  • Issue

  • 5