Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa.

Academic Article

Abstract

  • The scope of the present study was to evaluate the presence and activation of proacrosin/acrosin as a tool to determine the acrosomal status of fresh and frozen/thawed dog spermatozoa. Monoclonal antibody C5F11, directed against human acrosin, cross-reacted with dog spermatozoa and labeled the acrosome of both fresh and frozen/thawed dog spermatozoa. Frozen/thawed spermatozoa had a lesser proportion of labeled spermatozoa than fresh spermatozoa (P<0.05). When live spermatozoa were labeled with soybean trypsin inhibitor conjugated with Alexa 488 (SBTI-Alexa 488), the proportion of acrosome-labeled fresh spermatozoa was less than frozen/thawed spermatozoa (P<0.05). By using Western blots and enzymatic activity, frozen/thawed spermatozoa had a greater proportion of active acrosin than fresh spermatozoa. In addition, beta 1,4-galactosyl-transferase (GalT), a plasma membrane bound protein, remained attached to frozen/thawed spermatozoa. Proacrosin is activated during freezing/thawing of dog spermatozoa, and that proacrosin/acrosin may be a good indicator of acrosomal integrity of frozen/thawed spermatozoa.
  • Published In

    Keywords

  • Acrosin, Acrosome, Acrosome Reaction, Animals, Antibodies, Monoclonal, Blotting, Western, Dogs, Enzyme Precursors, Male, Semen Preservation, Spermatozoa
  • Digital Object Identifier (doi)

    Author List

  • Cortes CJ; Codelia VA; Manosalva I; de Lange J; De Los Reyes M; Moreno RD
  • Start Page

  • 165
  • End Page

  • 175
  • Volume

  • 93
  • Issue

  • 1-2