Synthesis of tumor necrosis factor-α mRNA in bronchoalveolar lavage cells from human immunodeficiency virus-infected persons with Pneumocystis carinii pneumonia

Academic Article

Abstract

  • Bronchoalveolar lavage fluid cells from a cohort of 34 human immunodeficiency virus-infected persons with established Pneumocystis carinii pneumonia were examined for expression of tumor necrosis factor-α (TNF-α) mRNA by fluorescence in situ hybridization with an antisense riboprobe. Video image analysis was used to develop a quantitative assay that evaluates relative single-cell levels of mRNA. The resulting data were analyzed as an antisense-to-sense ratio and examined for correlation between TNF-α mRNA expression and other measures of disease severity. Higher levels of TNF-α mRNA were seen in persons who had higher levels of arterial oxygen.
  • Authors

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    Digital Object Identifier (doi)

    Author List

  • Rayment N; Miller RF; Ali N; Binks MH; Katz DR
  • Start Page

  • 654
  • End Page

  • 659
  • Volume

  • 174
  • Issue

  • 3