Exosomal micro RNAs derived from dermal papilla cells mediate hair follicle stem cell proliferation and differentiation

Academic Article

Abstract

  • Recent studies have demonstrated that dermal papilla cell-derived exosomes (DPC-Exos) promote the anagen stage of hair follicle (HF) growth and delay the catagen stage. However, the roles of DPC-Exos in regulating hair follicle stem cell (HFSC) quiescence and activation remain unknown. Here, we found that HFSC differentiation was induced by co-culture with DPCs, and that DPC-Exos attached to the surface of HFSCs. Using micro RNA (miRNA) high-throughput sequencing, we identified 111 miRNAs that were significantly differentially expressed between DPC-Exos and DPCs, and the predicted target genes of the top 34 differentially expressed miRNAs indicated that DPC-Exos regulate HFSCs proliferation and differentiation via genes involved in cellular signal transduction, fatty acid expression regulation, and cellular communication. The overexpression of miR-22-5p indicated that it negatively regulates HFSC proliferation and LEF1 was revealed as the direct target gene of miR-22-5p. We therefore propose the miR-22-5p–LEF1 axis as a novel pathway regulating HFSC proliferation.
  • Authors

    Digital Object Identifier (doi)

    Author List

  • Yan H; Gao Y; Ding Q; Liu J; Li Y; Jin M; Xu H; Ma S; Wang X; Zeng W
  • Start Page

  • 1368
  • End Page

  • 1382
  • Volume

  • 15
  • Issue

  • 7