We report a precise measurement of the Ds± meson lifetime. The data were taken by the SELEX experiment (E781) spectrometer using 600 GeV/c ∑-, π- and p beams. The measurement has been done using 918 reconstructed Ds±. The lifetime of the Ds± is measured to be 472.5 ± 17.2 ± 6.6 fs, using K*(892)0K± and φπ± decay modes. The lifetime ratio of Ds± to D0 is 1.145 ± 0.049. © 2001 Elsevier Science B.V. All rights reserved.