Multiple immunizations with adenovirus and MVA vectors improve CD8+ T cell functionality and mucosal homing

Academic Article

Abstract

  • Recombinant adenovirus vectors and MVA vectors were used in prime boost vaccine regimens to address the impact of repeated immunizations on transgene product-specific CD8+ T cell frequencies, phenotypes, function, and localization. We show that a regimen with three immunizations incorporating MVA, human adenovirus serotype 5 and chimpanzee-derived adenoviruses serotype 68 or 7 yields high transgene product-specific CD8+ T cell frequencies in spleen, blood, lymph nodes, and peritoneal lavage. Furthermore, upon triple immunization increased frequencies of transgene-specific T cells were measured at mucosal sites such as mesenteric lymph nodes, intestinal epithelium, and Peyer's patches. Multiple dose vaccine regimens that markedly increase functionally active transgene-specific T cells and target them to the appropriate ports of entry may be important in protection against pathogens such as HIV-1. © 2007 Elsevier Inc. All rights reserved.
  • Authors

    Published In

  • Virology  Journal
  • Digital Object Identifier (doi)

    Author List

  • Tatsis N; Lin SW; Harris-McCoy K; Garber DA; Feinberg MB; Ertl HCJ
  • Start Page

  • 156
  • End Page

  • 167
  • Volume

  • 367
  • Issue

  • 1