Demonstration of circularization of herpes simplex virus DNA following infection using pulsed field gel electrophoresis

Academic Article

Abstract

  • Studies of the configuration of intracellular herpes simplex virus DNA have been limited by the inability of restriction enzyme analyses to distinguish circular DNA from other configurations. To address this issue, we used pulsed-field gel electrophoresis of virus-infected, cycloheximide-treated cells and detected accumulation of viral DNA that failed to migrate out of the sample wells of the gels. This DNA lacked terminal restriction fragments and could be converted to unit-length linear species by γ-irradiation, demonstrating the circularization of viral DNA following infection. © 1993 Academic Press, Inc.
  • Authors

    Published In

  • Virology  Journal
  • Digital Object Identifier (doi)

    Author List

  • Garber DA; Beverley SM; Coen DM
  • Start Page

  • 459
  • End Page

  • 462
  • Volume

  • 197
  • Issue

  • 1