Illumina Sequencing of Bisulfite-Converted DNA Libraries

Academic Article

Abstract

  • Here we describe a standard MethylC-seq protocol using single-read sequencing on an Illumina Genome Analyzer II platform. The protocol involves ligation of methylated sequencing adaptors to sonicated genomic DNA, gel purification, sodium bisulfite conversion, polymerase chain reaction (PCR) amplification, and sequencing.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Lizardi PM; Yan Q; Wajapeyee N
  • Volume

  • 2017
  • Issue

  • 11