Analysis of the transcriptional activity of retinoic acid-related orphan receptors (RORs) and inhibition by inverse agonists

Chapter

Abstract

  • Here, we describe several assays to analyze the transcriptional activity of retinoic acid-related orphan receptors (RORs) and the effect of inverse agonists on their activity. One assay measures the effect of an inverse agonist on the transcriptional activation of a luciferase reporter by RORs in a Tet-On cell system. A mammalian two-hybrid assay analyzes the interaction of the ROR ligand binding domain with a coactivator peptide. Two additional assays examine the effect of an inverse agonist on the activation of a luciferase reporter under control of the promoter of the ROR target gene, IL17, and on ROR-mediated activation using a mammalian monohybrid assay.
  • Digital Object Identifier (doi)

    Start Page

  • 193
  • End Page

  • 202
  • Volume

  • 1966