Intracellular calcium signaling regulates glomerular filtration barrier permeability: the role of the PKGIα-dependent pathway

Academic Article

Abstract

  • Podocytes are dynamic polarized cells that lie on the surface of glomerular capillaries and comprise an essential component of the glomerular filtration barrier. Insulin provoked a sustained, approximately 70%, increase in intracellular calcium concentration in podocytes. RT-PCR revealed the presence of mRNA encoding sarco/endoplasmic reticulum calcium ATPase isoforms 1–3, and plasma membrane Ca pump (PMCA) isoforms 1,3,4; mRNA levels were depressed by the addition of insulin. Inhibitors of PMCA, and the Na -Ca exchanger, increased podocyte permeability to albumin, induced dimerization of protein kinase G type I alpha (PKGIα), and activation of PKGIα-dependent signaling. These data suggest the involvement of calcium and PKGIα signaling in insulin-enhanced filtration barrier permeability in podocytes. 2+ + 2+
  • Published In

  • FEBS Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Piwkowska A; Rogacka D; Audzeyenka I; Kasztan M; Angielski S; Jankowski M
  • Start Page

  • 1739
  • End Page

  • 1748