Pharmacologically regulated gene expression in the retina following transduction with viral vectors

Academic Article

Abstract

  • The availability of inducible expression systems makes regulatable control of therapeutic proteins an attainable goal in gene therapy. We delivered tetracycline-inducible transgenes to the subretinal space using recombinant adenoviruses. Upon administration of doxycycline, we demonstrated reversible expression of green fluorescent protein in the retinal pigment epithelium as well as modulation of human growth hormone produced in the retina and secreted in the blood stream. This mode of delivery and regulation offers a unique way to evaluate gene function in the eye and represents a novel method for introducing therapeutic proteins into the retina.
  • Authors

    Published In

  • Gene Therapy  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 3530674
  • Author List

  • Dejneka NS; Auricchio A; Maguire AM; Ye X; Gao GP; Wilson JM; Bennett J
  • Start Page

  • 442
  • End Page

  • 446
  • Volume

  • 8
  • Issue

  • 6