Oligomeric structure of a simian virus 40 T antigen in free form and bound to DNA

Academic Article


  • The structure of the D2 protein, a polypeptide structurally and functionally related to the simian virus 40 large T antigen, was examined by electron microscopy. The observed size of the protein indicates that it most commonly exists as a tetrameric assembly of its 107,000 Mr monomer. In addition to the tetramers, both monomers and dodecamers are seen. Binding of D2 protein to a fragment of simian virus 40 DNA was found to occur preferentially at the origin of replication. The major binding species of the protein is the tetramer. © 1981.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Myers RM; Williams RC; Tjian R
  • Start Page

  • 347
  • End Page

  • 353
  • Volume

  • 148
  • Issue

  • 4