Computer-Assisted high-pressure liquid chromatography of radio-labelled phenylthiohydantoin amino acids

Academic Article


  • A computer-controlled high-pressure liquid chromatographic (HPLC) system is described to identify vitro phenyl [35S]isothiocyanate-labelled phenylthiohydantoin (PTH) amino acids from a solid-phase sequencer. Each radio-labelled amino acid from the sequencer is added to a PTH amino acid standard and the mixture separated by HPLC using a computer, programmed to detect a slope change in the absorbance. Individual fractions corresponding to the PTH amino acids are collected and counted. The sensitivity of the system is demonstrated on 700 pmoles of lysozyme. © 1978.
  • Authors

    Published In

    Digital Object Identifier (doi)

    Author List

  • Bhown AS; Mole JE; Hollaway WL; Bennett JC
  • Start Page

  • 35
  • End Page

  • 41
  • Volume

  • 156
  • Issue

  • 1