Tryptic glycopeptides from influenza A/WSN (HoN1) and mink cell focus (MCF)-inducing (MCF-247) murine leukemia virus were subjected to high performance liquid chromatography (hplc) for mapping purposes. Hydrophilic ion-pairing was accomplished using 0.1% phosphoric acid on C18 μBondapack and LiChrosorb RP-18 ODS commercially available columns. The samples were eluted from the column with acetonitrile with sample recovery in the range of 70 to 80%. The advantages of hplc over the conventional techniques previously used are detailed. © 1981, Taylor & Francis Group, LLC. All rights reserved.