Does the functional efficacy of skeletal myoblast transplantation extend to nonischemic cardiomyopathy?

Academic Article

Abstract

  • BACKGROUND: The benefits of skeletal myoblast (SM) transplantation on infarcted myocardium have been investigated extensively; however, little is known about its effects in nonischemic cardiomyopathy models. To address this issue, we tested SM transplantation in CHF147 Syrian hamsters, a strain characterized by a delta-sarcoglycan deficiency that phenotypically features the human setting of primary dilated cardiomyopathy. METHODS AND RESULTS: Cell culture techniques were used to prepare approximately 5x10(6) muscle cells from autologous tibialis anterior muscle, of which 50% were SMs (desmin staining). The cells were injected in 6 sites across the left ventricular wall (n=14). Control animals (n=12) received equivalent volumes of culture medium. Left ventricular systolic function was assessed in a blinded fashion from 2D echocardiographic left ventricular fractional area change, before transplantation, and 4 weeks later. Explanted hearts were processed for the detection of myotubes and quantification of fibrosis. Baseline functional data did not differ between the 2 groups. Four weeks after transplantation, 6 of the 10 surviving grafted hamsters were improved compared with 0 of the 8 survivors of the control group. This translated into a 6% decrease in fractional area change in controls compared with a 24% increase in cell-transplanted hamsters (P=0.001). Engrafted myotubes were consistently detected in all SM transplanted hearts by immunohistochemistry, whereas fibrosis was not worsened by cell injections. CONCLUSIONS: These data suggest that the functional benefits of SM transplantation might extend to nonischemic cardiomyopathy.

    • Authors

    Published In

  • Circulation  Journal

    • Keywords

  • Animals, Cardiomyopathy, Dilated, Cells, Cultured, Cricetinae, Female, Fibrosis, Heart Ventricles, Injections, Male, Mesocricetus, Muscle, Skeletal, Myoblasts, Myocardium, Random Allocation, Sarcoglycans, Single-Blind Method, Transplantation, Heterotopic, Ventricular Function, Left

    • Digital Object Identifier (doi)

    Pubmed Id

  • 19731989

    • Author List

  • Pouly J; Hagège AA; Vilquin J-T; Bissery A; Rouche A; Bruneval P; Duboc D; Desnos M; Fiszman M; Fromes Y

    • Start Page

  • 1626

    • End Page

  • 1631

    • Volume

  • 110

    • Issue

  • 12