Human melanoma metastasis is inhibited following ex vivo treatment with an antisense oligonucleotide to protein kinase C-α

Academic Article

Abstract

  • To determine whether alteration of PKCα expression would affect the metastatic potential of human melanoma cells, replicate cultures of C8161 cells were treated in vitro with a phosphorothioate antisense oligodeoxynucleotide (ODN) that specifically inhibits PKCα expression (ISIS-3521). Control C8161 cultures were treated with a scrambled sequence ODN, cationic liposomes or were left untreated. Northern blots demonstrated 70% inhibition of PKCα mRNA in ISIS-3521-treated cells compared to controls. Metastasis was suppressed by 75% when ISIS-3521-treated cells were injected intravenously into athymic mice. These results show that PKCα expression is important in the regulation of human melanoma metastasis.
  • Published In

  • Cancer Letters  Journal
  • Digital Object Identifier (doi)

    Author List

  • Dennis JU; Dean NM; Bennett CF; Griffith JW; Lang CM; Welch DR
  • Start Page

  • 65
  • End Page

  • 70
  • Volume

  • 128
  • Issue

  • 1