Fluorescent modification of adenosine 3′,5′-monophosphate: Spectroscopic properties and activity in enzyme systems

Academic Article


  • The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N6-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-nonophosphate. The potential utility of this analog is indicated by its long flouorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.
  • Published In

  • Science  Journal
  • Digital Object Identifier (doi)

    Pubmed Id

  • 10896725
  • Author List

  • Secrist JA; Barrio JR; Leonard NJ; Villar-Palasi C; Gilman AG
  • Start Page

  • 279
  • End Page

  • 280
  • Volume

  • 177
  • Issue

  • 4045