Leaves of Pterospermum acerifolium L. (Sterculiaceae) are used in India for reducing oxidative stress and inflammation. The objective of this study was to investigate the antioxidant and anti-inflammatory activities to justify the use of the plant in folkloric medicine. Antioxidant activity of different fractions were evaluated by using in-vitro antioxidant assays models like determination of total phenolics, DPPH radical scavenging assay, nitric oxide scavenging assay, hydroxy radical scavenging assay and superoxide anion scavenging assay. Anti-inflammatory activity was evaluated using carrageenan induced inflammation and thermally induced protein denaturation. Ethyl acetate fraction of P. acerifolium (EAF) showed highest free radical scavenging activity in all the models. EAF also produced significant anti-inflammatory activity in both in-vivo and in-vitro model. The results obtained in this study showed that the leaves of Pterospermum acerifolium L. have antioxidant and anti-inflammatory properties which provide a basis for the traditional use of the plant.