Cutting edge: Intracellular IFN-β and distinct type i IFN expression patterns in circulating systemic lupus erythematosus B cells

Academic Article

Abstract

  • Copyright © 2018 by The American Association of Immunologists, Inc. In systemic lupus erythematosus (SLE), type I IFNs promote induction of type I IFN-stimulated genes (ISG) and can drive B cells to produce autoantibodies. Little is known about the expression of distinct type I IFNs in lupus, particularly high-affinity IFN-β. Single-cell analyses of transitional B cells isolated from SLE patients revealed distinct B cell subpopulations, including type I IFN producers, IFN responders, and mixed IFN producer/responder clusters. Anti-Ig plus TLR3 stimulation of SLE B cells induced release of bioactive type I IFNs that could stimulate HEK-Blue cells. Increased levels of IFN-β were detected in circulating B cells from SLE patients compared with controls and were significantly higher in African American patients with renal disease and in patients with autoantibodies. Together, the results identify type I IFN-producing and -responding subpopulations within the SLE B cell compartment and suggest that some patients may benefit from specific targeting of IFN-β.
  • Published In

    Digital Object Identifier (doi)

    Pubmed Id

  • 12808323
  • Author List

  • Hamilton JA; Wu Q; Yang PA; Luo B; Liu S; Li J; Mattheyses AL; Sanz I; Chatham WW; Hsu HC
  • Start Page

  • 2203
  • End Page

  • 2208
  • Volume

  • 201
  • Issue

  • 8