Chemical cross-linking of ethidium to DNA by glyoxal

Academic Article


  • Ethidium was found to be efficiently cross-linked to DNA by glyoxal. Kinetic studies showed that the rate of the cross-linking reaction is strongly dependent on the glyoxal concentration. Comparative studies using a series of phenanthridines and acridines showed that NH2 groups at both the 2 and 7 positions on the phenanthridine ring are necessary for efficient cross- linking. Studies using synthetic polydeoxynucleotides showed that the 2- amino group of guanine is absolutely required for cross-linking. Fluorescence contact energy transfer and relative viscosity experiments showed that the cross-linked drug remains intercalated into DNA. DNA gel electrophoresis and melting studies demonstrated that cross-linked ethidium does not dissociate the DNA double helix to single strands.
  • Digital Object Identifier (doi)

    Pubmed Id

  • 14662304
  • Author List

  • Leng F; Graves D; Chaires JB
  • Start Page

  • 71
  • End Page

  • 81
  • Volume

  • 1442
  • Issue

  • 1