The deficiency of Gαq leads to enhanced T-cell survival

Academic Article

Abstract

  • We have previously reported that G , the α subunit of the G protein, had important roles in dendritic cell migration, B-cell survival and autoimmunity. In this study, we showed that the deficiency of G led to enhanced T-cell survival. Cultured Gnaq T cells exhibited survival advantages both in medium alone and in the presence of anti-CD3 stimulation. Gnaq T cells still exhibited a survival advantage when they were cultured in the presence of interleukin (IL)-2 or IL-7. Gnaq T cells were more resistant to activation-induced cell death (AICD) in vitro. The survival advantage of Gnaq T cells was further confirmed by transferring T cells into syngeneic hosts in vivo. G deficiency might promote T-cell survival by upregulated Bcl-x L expression and downregulated Fas and FasL expressions. Furthermore, upon T-cell receptor (TCR) ligation, Akt activity was increased in Gnaq T cells in comparison with wild-type (WT) T cells. The survival advantage of Gnaq T cells was significantly attenuated after adding Akt inhibitor. Taken together, our data demonstrated a negative role of G in regulating T-cell survival. αq q αq αq αq -/- -/- -/- -/- -/- -/-
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Wang D; Zhang Y; He Y; Li Y; Lund FE; Shi G
  • Start Page

  • 781
  • End Page

  • 790
  • Volume

  • 92
  • Issue

  • 9