Gravidas with class III obesity: evaluating the abdominal skin microbiota above and below the panniculus*

Academic Article


  • Objective: We sought to compare the skin microbiota above and below the panniculus of third-trimester pregnant women with class III obesity. Methods: We collected swabs from the anterior panniculus and from the intertriginous area under the panniculus of women with class III obesity (body mass index ≥40 kg/m2) carrying a live singleton at ≥28 weeks. DNA was extracted. PCR with primers targeting the 16S rDNA V4 region were used to prepare an amplicon library. PCR products were sequenced (Illumina MiSeq platform). The 16S rDNA sequences were processed, integrated, analyzed and reported using QIIME and in-house developed scripts. Taxonomy was assigned using RDP Classifier (threshold 0.8) against the Greengenes 16S rDNA database. Results: Twenty women were enrolled. One sample pair was excluded for low sequence depth. Using permutation testing, there was significantly less bacterial diversity in the samples above compared to below the panniculus (beta diversity using weighted UniFrac metrics; p < 0.001). Conclusion: In pregnant women with class III obesity, the skin microbiota in the subpannicular fold differs from and is more diverse than that on the anterior panniculus. © 2015 Taylor & Francis.
  • Keywords

  • Intertriginous microbiome morbid obesity pregnancy skin flora DNA 16S Actinobacteria Article Bacteroidetes body height body mass body weight Brevibacterium cesarean section cohort analysis Comamonadaceae controlled study Corynebacterium DNA extraction female Firmicutes gestational age human maternal obesity Paenibacillus panniculus carnosus polymerase chain reaction pregnancy diabetes mellitus pregnant woman Prevotella priority journal prospective study Proteobacteria risk factor skin biopsy Staphylococcus Streptococcus taxonomy third trimester pregnancy weight gain abdominal subcutaneous fat adult comparative study microbiology microflora pregnancy complication skin young adult Humans Microbiota Obesity, Morbid Pregnancy Complications Prospective Studies Subcutaneous Fat, Abdominal
  • Digital Object Identifier (doi)

    Author List

  • Edwards RK; Kumar R; Zhi D; Szychowski J; Subramaniam A; Lefkowitz EJ; Morrow C
  • Start Page

  • 3312
  • End Page

  • 3316
  • Volume

  • 29
  • Issue

  • 20