The Golgi association of endothelial nitric oxide synthase is necessary for the efficient synthesis of nitric oxide

Academic Article

Abstract

  • The particulate enzyme, endothelial nitric oxide synthase (eNOS), produces nitric oxide to maintain normal vasodilator tone in blood vessels. In this study, we demonstrate that eNOS is a Golgi-associated protein in cultured endothelial cells and intact blood vessels. Using a heterologous expression system in HEK 293 cells, we show that wild-type myristoylated and palmitoylated eNOS, but not mutant, non-acylated eNOS targets to the Golgi. More importantly, HEK 293 cells expressing wild-type eNOS release substantially more NO than cells expressing the mutant, non-acylated enzyme. Thus, eNOS is a novel Golgi-associated protein, and Golgi compartmentalization is necessary for the enzyme to respond to intracellular signals and produce NO.
  • Published In

    Digital Object Identifier (doi)

    Author List

  • Sessa WC; Garcia-Cardena G; Liu J; Keh A; Pollock JS; Bradley J; Thiru S; Braverman IM; Desai KM
  • Start Page

  • 17641
  • End Page

  • 17644
  • Volume

  • 270
  • Issue

  • 30