Disassembly of the nuclear envelope of spisula oocytes in a cell-free system

Academic Article


  • Nuclei isolated from oocytes of the surf clam Spisula solidissima are disassembled when exposed to extracts from maturing oocytes. In the course of this process the nuclear lamina undergoes a marked reduction in size and the nuclear membrane appears to be fragmented into vesicles. These events are accompanied by extensive phosphorylation of the oocyte 67-kDa lamin and its solubilization. The changes observed are similar to those which occur in vivo in activated Spisula oocytes. Nuclear envelope breakdown in vitro requires ATP and Mg2+, but not Ca2+. It is not affected by protease inhibitors and is inhibited by alkaline phosphatase. © 1989 Academic Press, Inc.
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    Digital Object Identifier (doi)

    Author List

  • Dessev G; Palazzo R; Rebhun L; Goldman R
  • Start Page

  • 496
  • End Page

  • 504
  • Volume

  • 131
  • Issue

  • 2